Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.053804 |
Chromosome: | chromosome_17 |
Location: | 3899260 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g728300 | FAP226,CAL6 | Calpain cysteine protease and Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AACGCCTCTGCCTGTGCTCCGCTCGTCTTC |
Internal bar code: | TAACCTGACCTTAGATACGGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 569 |
LEAP-Seq percent confirming: | 99.5652 |
LEAP-Seq n confirming: | 1374 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTGAACAGGCAGAACAGC |
Suggested primer 2: | GCAAATACCAGCCCGAACTA |