| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053811 |
| Chromosome: | chromosome 8 |
| Location: | 1280563 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363500 | (1 of 2) 4.2.1.134 - Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGATGGTGGAAACAAGCCCCCCGCCCC |
| Internal bar code: | GTGATTCAGCTCACCGTGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1050 |
| LEAP-Seq percent confirming: | 99.6702 |
| LEAP-Seq n confirming: | 11786 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCACATACACCGCTCTC |
| Suggested primer 2: | ACCTCTCACCTCTCACCCCT |