| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.053822 |
| Chromosome: | chromosome 16 |
| Location: | 2349692 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659667 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGTGGCCTGCAGGCGTTTGGACGGATG |
| Internal bar code: | ACGTCATCCGAAGTGTCTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.2151 |
| LEAP-Seq n confirming: | 5941 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGAAGTCTAGGTCAGGG |
| Suggested primer 2: | GAGAGACTCGAAACCGATGC |