| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.053858 |
| Chromosome: | chromosome 17 |
| Location: | 4192231 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730100 | RSE1,RSEP1 | (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGCGACAGCTTTCGAGTAGGGTTGTAG |
| Internal bar code: | CCTTTGCTCTTGTGTACACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.2698 |
| LEAP-Seq n confirming: | 2855 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGGCTATGTCACCTTCC |
| Suggested primer 2: | GAACAACAGCGTTCAGGGAT |