| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.053881 |
| Chromosome: | chromosome_10 |
| Location: | 675733 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g422250 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGTCGCACAAGGAGTGGGCACCGGTGGGG |
| Internal bar code: | GAGGGATGGAAGGCGGGGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 99.6393 |
| LEAP-Seq n confirming: | 5248 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATAGCACAGAGGCTGGTG |
| Suggested primer 2: | CTGTGTCTTGTGCGTGTGTG |