Insertion junction: LMJ.RY0402.053904_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.053904
Chromosome:chromosome 17
Location:4169157
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g729950 SRX1,SRX2 (1 of 2) 1.8.98.2 - Sulfiredoxin / Peroxiredoxin-(S-hydroxy-S-oxocysteine) reductase; Related to sulfiredoxin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACATTGCGCACACTGACATGCGCGCGTGC
Internal bar code:GGTGGAAATCGTAAAAAGGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:709
LEAP-Seq percent confirming:99.6564
LEAP-Seq n confirming:1160
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGAAACAGGGGTGAAACT
Suggested primer 2:CAAGCTGCTTTGGTAGAGGG