| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053908 |
| Chromosome: | chromosome 6 |
| Location: | 6499922 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293100 | (1 of 2) PTHR19957:SF124 - SYNTAXIN-8B-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTGAAGGGCACAGACTGGCAGACATGC |
| Internal bar code: | CTGCCGCAGGGAGCAAGCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.8277 |
| LEAP-Seq n confirming: | 8691 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTAGCTCAGTCAAGGTG |
| Suggested primer 2: | GGGTGTTGAAATGCTTTGCT |