Insertion junction: LMJ.RY0402.053909_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.053909
Chromosome:chromosome 1
Location:5191484
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g036100 (1 of 1) K11790 - denticleless (DTL, CDT2, DCAF2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACGTGTGCGCGTGGACTGATTGATGAC
Internal bar code:CAGCTGTTAATAAACTTTAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:643
LEAP-Seq percent confirming:99.5605
LEAP-Seq n confirming:4304
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CATGACACGGCATCCTAATG
Suggested primer 2:CTGATGCTGATGACGACGAT