| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053909 |
| Chromosome: | chromosome_6 |
| Location: | 3761382 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278157 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGCGTGCGGCTACAATTAGCTAGCCGCAA |
| Internal bar code: | CTTCACTAATTGTCATGTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1195 |
| LEAP-Seq percent confirming: | 99.579 |
| LEAP-Seq n confirming: | 5913 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTATGTGGGCCATTGCTT |
| Suggested primer 2: | TCTTCTCACCAGCTCCACCT |