| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.053913 |
| Chromosome: | chromosome 16 |
| Location: | 6314484 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674500 | ABCA4 | ABC transporter 4; (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGCTTGGCTCTCCCACCGCACCCTCAA |
| Internal bar code: | GGGCGCGTTATTCGGGGCATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 284 |
| LEAP-Seq percent confirming: | 74.5894 |
| LEAP-Seq n confirming: | 772 |
| LEAP-Seq n nonconfirming: | 263 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAATATGAGCTGCTGCGG |
| Suggested primer 2: | GTTTCGTTTGTGACGGTGTG |