Insertion junction: LMJ.RY0402.053936_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.053936
Chromosome:chromosome 1
Location:3056787
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g018850 EEP2 (1 of 4) K12603 - CCR4-NOT transcription complex subunit 6 (CNOT6, CCR4); Exonuclease-Endonuclease-Phosphatase superfamily protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTAGTATGATAACTCGACTCGGCAGGTCG
Internal bar code:GCATTGGATTATCGTCGCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:744
LEAP-Seq percent confirming:96.0073
LEAP-Seq n confirming:1058
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGTAGTCCAGACAGCCC
Suggested primer 2:TCAAAACTTGACAGGGGGTC