| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053936 |
| Chromosome: | chromosome 1 |
| Location: | 3056825 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018850 | EEP2 | (1 of 4) K12603 - CCR4-NOT transcription complex subunit 6 (CNOT6, CCR4); Exonuclease-Endonuclease-Phosphatase superfamily protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCCCTCCCGCACATGCACACGTACGCA |
| Internal bar code: | GGGATTGATCACTTACTACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 99.3827 |
| LEAP-Seq n confirming: | 644 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTCGTACACGCCACTGA |
| Suggested primer 2: | TCAAAACTTGACAGGGGGTC |