| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053978 |
| Chromosome: | chromosome 12 |
| Location: | 1190324 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489800 | (1 of 4) PF00887 - Acyl CoA binding protein (ACBP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATCTCGCCACCCTGCCCTTACCGGCTT |
| Internal bar code: | TAAACTCTCGCAGGTTACATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 219 |
| LEAP-Seq percent confirming: | 99.6976 |
| LEAP-Seq n confirming: | 989 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCCTTCCAGGCCAGTAT |
| Suggested primer 2: | GCTGACATTTGAGGAGGAGC |