| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054008 |
| Chromosome: | chromosome 2 |
| Location: | 5477300 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108000 | (1 of 1) K16315 - serine/threonine-protein kinase haspin [EC:2.7.11.1] (GSG2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCGGCAAGCGGCGCTTGCGGGGTTGCG |
| Internal bar code: | GCAGGTCACGCCTCGGTACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 99.6241 |
| LEAP-Seq n confirming: | 265 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTAATGCCCTGCAGAATG |
| Suggested primer 2: | AATGGGCACCACCTTGATAA |