| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054053 |
| Chromosome: | chromosome 17 |
| Location: | 2534421 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716451 | (1 of 1) 1.14.11.13 - Gibberellin 2-beta-dioxygenase / Gibberellin 2-beta-hydroxylase; Similar to plant Gibberellin 2-beta-dioxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGGGCCGGACTGCGAGCAGCCTCGAGC |
| Internal bar code: | CCCTGCAACTACTCCGCTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 99.6468 |
| LEAP-Seq n confirming: | 12695 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCGGCTCTAGTTTTGAC |
| Suggested primer 2: | AGCTACTCAGCTTTCCGCTG |