Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.054094 |
Chromosome: | chromosome_13 |
Location: | 2379495 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g579450 | CST1 | Chlamy-specific membrane transporter of unknown function | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCAGATGAGCGACACCTCCCTGACCACCG |
Internal bar code: | AATTGTGAGGAAGGACGAATTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 950 |
LEAP-Seq percent confirming: | 99.1326 |
LEAP-Seq n confirming: | 4000 |
LEAP-Seq n nonconfirming: | 35 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGACCTCGCTGTTGACTTTG |
Suggested primer 2: | AACCCACGCAATTTGAACTC |