Insertion junction: LMJ.RY0402.054095_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.054095
Chromosome:chromosome 3
Location:9043180
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g210513 (1 of 2) K05894 - 12-oxophytodienoic acid reductase (OPR) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCACCGGCGCGGAGCCGTCGCCCAGGTC
Internal bar code:GTAGGAACCGTCGTCGATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:929
LEAP-Seq percent confirming:99.2823
LEAP-Seq n confirming:5395
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:ATATCACGCCCAAATCAAGC
Suggested primer 2:GAAAGCTTCAACAAGCAGGG