| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054125 |
| Chromosome: | chromosome 12 |
| Location: | 1566265 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486700 | FAP271 | (1 of 7) PF07004 - Sperm-tail PG-rich repeat (SHIPPO-rpt); Flagellar Associated Protein 271 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCTGCGAAGGGGAGGAAGTGCCCCGCA |
| Internal bar code: | GCGCGTCACGAGGTAAAGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 97.5451 |
| LEAP-Seq n confirming: | 5682 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGGTTCTGAGTCTCTCTG |
| Suggested primer 2: | CTAAGGAAAGCGACACGAGG |