Insertion junction: LMJ.RY0402.054202_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.054202
Chromosome:chromosome 2
Location:3311656
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g095087 (1 of 2) 3.4.14.1 - Dipeptidyl-peptidase I / Dipeptidyl transferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCGGCAACAACGGAAACGGAAACGGAAA
Internal bar code:ACGGGTCGTGGGCAGGAGCCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:231
LEAP-Seq percent confirming:74.5455
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGAAACGTGCTGTCTGTT
Suggested primer 2:CAGCATCGTCAAGAACAGGA