| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054208 |
| Chromosome: | chromosome 3 |
| Location: | 7701024 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203700 | DEGO1,DEG12 | DegP-type protease-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACTGGTGCTAGCAACGCACTCATTCCC |
| Internal bar code: | AGGACGTGGCGGGCAGCGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 99.898 |
| LEAP-Seq n confirming: | 2939 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTGGAAAGCTTATGGGCG |
| Suggested primer 2: | GATGTTTGGCTGGGAAGAAA |