| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054216 |
| Chromosome: | chromosome 1 |
| Location: | 3741506 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024100 | (1 of 19) IPR018392 - LysM domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGGGGTGATCTTGACGCTGCACTCCT |
| Internal bar code: | GGGTAAAATACAGGGCCGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 301 |
| LEAP-Seq percent confirming: | 99.763 |
| LEAP-Seq n confirming: | 4631 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAGGTAGGTGGGCACTG |
| Suggested primer 2: | GCCAAATGTTACCGCAAACT |