Insertion junction: LMJ.RY0402.054216_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.054216
Chromosome:chromosome 1
Location:3741618
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g024100 (1 of 19) IPR018392 - LysM domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATCCCTACACCCTCTCTTGATGTCACAC
Internal bar code:GCGCAACGTACTTGCGTCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1116
LEAP-Seq percent confirming:99.5838
LEAP-Seq n confirming:1914
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AAACAGGTAGGTGGGCACTG
Suggested primer 2:GCCAAATGTTACCGCAAACT