Insertion junction: LMJ.RY0402.054233_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.054233
Chromosome:chromosome 3
Location:7330246
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g206650 (1 of 1) PTHR24349:SF88 - SERINE/THREONINE-PROTEIN KINASE CG17528 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGAGGCAGAGCTGGGAGCGCTGGTATTC
Internal bar code:CAGGAGTACCATAAGTCACCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:900
LEAP-Seq percent confirming:99.7846
LEAP-Seq n confirming:2779
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCTATTCGTCGACTTGTGCG
Suggested primer 2:GTCAGACCGCCTAGCTTCAC