| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054234 |
| Chromosome: | chromosome 14 |
| Location: | 2650393 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626350 | (1 of 1) PF04751 - Protein of unknown function (DUF615) (DUF615) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAAACCCGGGCCGCCGTGGCGGGGCACG |
| Internal bar code: | CCCGGCAAGCCATGCCATTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 273 |
| LEAP-Seq percent confirming: | 80.5422 |
| LEAP-Seq n confirming: | 2347 |
| LEAP-Seq n nonconfirming: | 567 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACGCCTCCTATCAACC |
| Suggested primer 2: | CTCTCTTCCCCTCCCATTTC |