Insertion junction: LMJ.RY0402.054240_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.054240
Chromosome:chromosome 3
Location:7884747
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g202500 (1 of 1) PF04836//PF05004 - Interferon-related protein conserved region (IFRD_C) // Interferon-related developmental regulator (IFRD) (IFRD) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTTTGGCTTCCAGCCGCGCGCGGAGCGG
Internal bar code:GAAGAGGGTCCGTAGGAGGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:417
LEAP-Seq percent confirming:98.806
LEAP-Seq n confirming:331
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGAAGGTTGCAAGAGGC
Suggested primer 2:TTAACACTTAGGTTGGGCGG