| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054268 |
| Chromosome: | chromosome 11 |
| Location: | 3135362 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479200 | (1 of 5) PF00995 - Sec1 family (Sec1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGCCCTGCAAGCTGCTGGAGCATTCGT |
| Internal bar code: | TGCCGGGCCCCTGCCAACGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 98.023 |
| LEAP-Seq n confirming: | 4859 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCGTAGTCCTTGCCACC |
| Suggested primer 2: | GAACTGCTGAAGGTCTTGCC |