| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.054288 |
| Chromosome: | chromosome 17 |
| Location: | 6606365 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g743547 | (1 of 16) IPR003439//IPR003593//IPR013525//IPR027417 - ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAAACCGCGCTTTCCCCTGCTCCGGCA |
| Internal bar code: | TCTTTTCCCTGTGTGCGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1151 |
| LEAP-Seq percent confirming: | 99.5169 |
| LEAP-Seq n confirming: | 3090 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATAGATGCACAGGCGCAG |
| Suggested primer 2: | GTGTACTTCATGGCGGGACT |