| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054319 |
| Chromosome: | chromosome 6 |
| Location: | 7705258 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAATTGATGTCACCCCATATAATATTTT |
| Internal bar code: | TGGCTACATGCGCACTTCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1200 |
| LEAP-Seq percent confirming: | 99.3702 |
| LEAP-Seq n confirming: | 6627 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATTGCGTAGCATCGTAGC |
| Suggested primer 2: | GCTCTTTGGACTTGTAGCGG |