| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054351 |
| Chromosome: | chromosome 9 |
| Location: | 2451640 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391370 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCAATGCCGCGGTGGTGTTGAACCGTT |
| Internal bar code: | CAGCACAAAAACCTCGCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 334 |
| LEAP-Seq percent confirming: | 98.8764 |
| LEAP-Seq n confirming: | 440 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGATCAAACAGCAGGGTT |
| Suggested primer 2: | TCTGCAGTGTAGTGCCCTTG |