| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.054369 |
| Chromosome: | chromosome 1 |
| Location: | 7496516 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053900 | (1 of 1) K01456 - peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase (E3.5.1.52, NGLY1, PNG1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAGGGACGGACGGGGCCTCAGGGCAAG |
| Internal bar code: | CGTGAGGGGAACAGCAGATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 30 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGGGGTTGAAACAGATG |
| Suggested primer 2: | GTGCTCGCTGAAAGTAAGGG |