| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054400 |
| Chromosome: | chromosome 12 |
| Location: | 7935823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTCGCAGTTGTGCGCTGCAATCACCATT |
| Internal bar code: | TCTAAGTTTGGGTTTGAATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 99.5229 |
| LEAP-Seq n confirming: | 1043 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTATGGGCAAGATGCAG |
| Suggested primer 2: | TCAGAGACCTTGACCTGCCT |