| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054424 |
| Chromosome: | chromosome 1 |
| Location: | 2207715 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012050 | NAR1F | Formate/nitrite transporter; (1 of 7) PTHR30520 - FORMATE TRANSPORTER-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGAGGCTTCAAGTTTTCAACGGATCTA |
| Internal bar code: | TGAAAAGACAGGTCACTTAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 99.6667 |
| LEAP-Seq n confirming: | 299 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGGTGTGGAGATAAGGA |
| Suggested primer 2: | CACACGGTACGATGATTGGA |