| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054447 |
| Chromosome: | chromosome_10 |
| Location: | 4627470 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g452950 | TRP1 | Transient receptor potential ion channel protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCAACACGAGTAACGTCAGCAGGACCACG |
| Internal bar code: | CGCTCTTATGGGGCGTCGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 99.2129 |
| LEAP-Seq n confirming: | 2269 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGCGTTACCGACTACAC |
| Suggested primer 2: | GGATTGGAAGGGGGTTACAT |