| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054457 |
| Chromosome: | chromosome 1 |
| Location: | 6684876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048000 | TPM1 | Tropomyosin 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTCGAGCGGCTGGAGGCGATTGCCCGC |
| Internal bar code: | TATGTAACTTCCATTGCGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 99.5386 |
| LEAP-Seq n confirming: | 1510 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGCAGGAGTACGTTGT |
| Suggested primer 2: | GCACCAAGAGGAGTCAAAGC |