| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054457 |
| Chromosome: | chromosome 1 |
| Location: | 6684879 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048000 | TPM1 | Tropomyosin 1 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCACGTGAAGGCCGTTCGCGTTACCG |
| Internal bar code: | TCGTCCCTGGACTTCCGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 793 |
| LEAP-Seq percent confirming: | 98.8674 |
| LEAP-Seq n confirming: | 2357 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGCAGGAGTACGTTGT |
| Suggested primer 2: | GCACCAAGAGGAGTCAAAGC |