| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054471 |
| Chromosome: | chromosome 16 |
| Location: | 1150102 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650350 | (1 of 1) PF03457 - Helicase associated domain (HA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCACCGCCGCCCACAGATGCCGCCGTCC |
| Internal bar code: | GCACGCGGCCACGTGGGTAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 99.1425 |
| LEAP-Seq n confirming: | 2775 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCTCCGTAGCTCCGTTA |
| Suggested primer 2: | ACCGTTGATGTGGAGAAAGG |