| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054485 |
| Chromosome: | chromosome 9 |
| Location: | 5248875 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399626 | (1 of 1) K09919 - hypothetical protein (K09919) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTTGAGCCTTAGTCCTCTTAATTACGC |
| Internal bar code: | GTGGGCTAACGGCGCGGGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 72.5 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGCAGCCTGTCATCGTC |
| Suggested primer 2: | AGTACGTGCGTGTCACTTGC |