| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054494 |
| Chromosome: | chromosome 1 |
| Location: | 2549372 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014751 | (1 of 7) PF14555 - UBA-like domain (UBA_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGGAACGGCCGGGCCAGGGCCCCTGGT |
| Internal bar code: | GAAAGTAGAATGCTAAAGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 99.6943 |
| LEAP-Seq n confirming: | 2283 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTGGCTAGGACTCACTG |
| Suggested primer 2: | CTCAATGGGCCACTGGTACT |