| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054500 |
| Chromosome: | chromosome 1 |
| Location: | 3485948 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022150 | KCN3 | Voltage-gated K+ channel; (1 of 1) PTHR11537//PTHR11537:SF158 - VOLTAGE-GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGTGTTGCAATGAGCGCACCGGCATT |
| Internal bar code: | TAGCTTTCGTAGTATTAGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 99.8514 |
| LEAP-Seq n confirming: | 3359 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCATACCGCTCACCATC |
| Suggested primer 2: | AAGCAAACATGTACCCTGCC |