| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054534 |
| Chromosome: | chromosome 5 |
| Location: | 1065614 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246150 | CYG13 | Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAGCGCCAGGCAGTGTGTTTAGTAGCA |
| Internal bar code: | CCGTAAAGTGCACCTTGGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.8717 |
| LEAP-Seq n confirming: | 3114 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGTACATGGAGGGTGGT |
| Suggested primer 2: | GACGAAAGCTGTGTCAACGA |