| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054536 |
| Chromosome: | chromosome 16 |
| Location: | 5936090 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677000 | HSP70E,GEX27 | (1 of 1) K09489 - heat shock 70kDa protein 4 (HSPA4); Heat shock protein 70E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTGCGCTGTGCTGTGGCGCCGCGCCT |
| Internal bar code: | GCCGAAGAGCCCGTAGTGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 99.6107 |
| LEAP-Seq n confirming: | 1791 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGAACATACTGGGGTG |
| Suggested primer 2: | TGTGAGGGTTTGTGCAACAT |