| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054545 |
| Chromosome: | chromosome 16 |
| Location: | 750652 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690950 | (1 of 54) IPR011047 - Quinonprotein alcohol dehydrogenase-like superfamily | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCTTCACGAGCTACACAAAGCCCTGGC |
| Internal bar code: | CCAATTGGCAAATGGCAGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 99.6642 |
| LEAP-Seq n confirming: | 2968 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGCTTGTAGGCTGGAGG |
| Suggested primer 2: | TGAGCAGCGGTTACTCACAC |