Insertion junction: LMJ.RY0402.054547_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.054547
Chromosome:chromosome 7
Location:5921032
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g354100 HAT4 (1 of 1) K07739 - elongator complex protein 3 [EC:2.3.1.48] (ELP3, KAT9); Histone acetyltransferase 4 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGTCCAGGTATTCAGCGCTCGCGCCGGC
Internal bar code:GTAGAATTAAGGAGGCTGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:987
LEAP-Seq percent confirming:99.3554
LEAP-Seq n confirming:11715
LEAP-Seq n nonconfirming:76
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:AACTACTCCGTTTGGCCCTT
Suggested primer 2:AGAATTGTATTTGGCCACGC