| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054552 |
| Chromosome: | chromosome 4 |
| Location: | 1838590 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g211650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCAATGAGGTGGTGGTGCCGCAGGGAG |
| Internal bar code: | TGAGTCAATTTTGTATGCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 99.6547 |
| LEAP-Seq n confirming: | 32613 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCCTGGCTTACGTGGAC |
| Suggested primer 2: | GTGTGAGGGTTCGTCCAGAT |