| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054588 |
| Chromosome: | chromosome 16 |
| Location: | 5994464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676600 | (1 of 4) IPR029041 - FAD-linked oxidoreductase-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGTGCGTGTGCCGGGGAGGACTGAAA |
| Internal bar code: | GACTGACGTGCCAAAACTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 99.3322 |
| LEAP-Seq n confirming: | 4760 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTGTCGTTCTGGGTCAG |
| Suggested primer 2: | TTTCTACGTGCATTCAAGCG |