| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054590 |
| Chromosome: | chromosome 16 |
| Location: | 1899690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655900 | (1 of 5) IPR007213//IPR029063 - Leucine carboxyl methyltransferase // S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCAGCGGCTGCGCCACCCGAGAGCTCT |
| Internal bar code: | ACCTTGATGTTACTAGAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 98.9043 |
| LEAP-Seq n confirming: | 1715 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCATAGCATCCACACCTG |
| Suggested primer 2: | AGGAAACTGGCTGTTCGCTA |