| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.054593 |
| Chromosome: | chromosome 1 |
| Location: | 1056440 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g006150 | EFG9,PRFC1, PRFC1,PRF3 | (1 of 1) PTHR23115//PTHR23115:SF69 - TRANSLATION FACTOR // PEPTIDE CHAIN RELEASE FACTOR 3; Putative chloroplast translation peptide-chain release factor 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACTGGTCTTCAAGCTGCAGGTGCGGAG |
| Internal bar code: | CCACGGTCATTTGCAGACCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 90.303 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCCTCCCACATCTACCT |
| Suggested primer 2: | CTCCAAGTCCGAGTACGAGC |