| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054598 |
| Chromosome: | chromosome 1 |
| Location: | 824160 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004157 | (1 of 28) PTHR31600//PTHR31600:SF2 - FAMILY NOT NAMED // TINY MACROCYSTS PROTEIN B-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCTTGAGAGGGTGCAAAAGGAGCGCG |
| Internal bar code: | GAAACGGGCCTCCTTTTGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1461 |
| LEAP-Seq percent confirming: | 99.035 |
| LEAP-Seq n confirming: | 12521 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTTCCTGTGGGTCATCT |
| Suggested primer 2: | ATGCAGTGCTCAATGCTACG |