| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054615 |
| Chromosome: | chromosome 14 |
| Location: | 2973777 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627850 | (1 of 2) 1.17.1.8 - 4-hydroxy-tetrahydrodipicolinate reductase / Dihydrodipicolinate reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCAGGATAGATCCAGCTGCCGGCCCCA |
| Internal bar code: | CACGATCGATGATCGGCTTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 99.5043 |
| LEAP-Seq n confirming: | 803 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCAGGCTTGCTACCAG |
| Suggested primer 2: | GAGCTTCGTGTCTGTGTGGA |