| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054616 |
| Chromosome: | chromosome 17 |
| Location: | 3125614 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721450 | NSS4 | (1 of 1) PTHR11819//PTHR11819:SF166 - SODIUM/SOLUTE SYMPORTER // SUBFAMILY NOT NAMED; Sodium:solute symporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCGCGTGCTTCAGCCTGGTGGGCATG |
| Internal bar code: | GAGCTGCCTCAGTCGAGTAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 99.4818 |
| LEAP-Seq n confirming: | 5183 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCGCTCATCATCATCTC |
| Suggested primer 2: | CTAAAGCAAGACCCAGCCAG |